Biodiversity and Ecophysiology of Yeasts by Marc-André Lachance (auth.), Dr. Gábor Péter, Professor

By Marc-André Lachance (auth.), Dr. Gábor Péter, Professor Carlos Rosa (eds.)

In the previous few many years an increasing number of yeast habitats that weren't investigated previous, spanning chilly climates to tropical areas and dry deserts to rainforests, were explored. hence, a wide physique of ecological facts has been amassed and the variety of recognized yeast species has elevated swiftly. This e-book offers an summary of the biodiversity of yeasts in numerous habitats. the hot advances completed by way of the appliance of molecular organic equipment within the box of yeast taxonomy and ecology also are integrated within the e-book. anywhere attainable, the interplay among yeasts and the encompassing setting is discussed.

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Int J Syst Bacteriol 49:309–318 Sampaio JP (2004) Diversity, phylogeny and classification of basidiomycetous yeasts. In: Agerer R, Blanz P, Peipenbring F (eds) Frontiers in basidiomycete mycology. Springer, Berlin Heidelberg New York, pp 49–80 29 30 Cletus P. Kurtzman and Jack W. Fell Sampaio JP, Gadanho M, Santos S, Duarte FL, Pais C, Fonseca A, Fell JW (2001) Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium: Rhodosporidium kratochvilovae and related anamorphic species. Int J Syst Evol Microbiol 51:687–697 Sampaio JP, Golubev WI, Fell JW, Gadanho M, Golubev MV (2004) Curvibasidium cygneicollum gen.

The BioloMICS software also allows for online polyphasic similarity-based identifications, which can be performed using any combination of geographic, morphological, physiological or 41 42 Vincent Robert et al. molecular data. The CBS in The Netherlands, CABI in the UK and Landcare Research in New Zealand have launched the Mycobank website (Crous et al. org) that allows the online registration of new fungal names as well as associated data. For the yeasts, this website is coupled to the yeast BioloMICS database and new species are made available online directly.

Multiplex PCR of larger numbers of primers in a single reaction is difficult owing to various factors, such as formation of primer dimers and differences in temperature requirements for primer hybridizations. Our laboratories have been exploring a high-throughput probe hybridization method for detection of multiple species in multiple samples. The method (Diaz and Fell 2004; Page and Kurtzman, 2005) is an adaptation of the Luminex xMAP technology (Luminex Corporation), which consists of a combination of 100 different sets of fluorescent beads covalently bound to species-specific capture probes.

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