By C.C. Reddy (Eds.)
The second one of 2 volumes proposing present examine into oxidation structures, this ebook is meant for biochemists, toxicologists, and pharmacologists. subject matters mentioned contain oxidation mechanisms in carcinogenesis, lipid peroxidation and different non-enzymatic reactions of oxygen
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Additional info for Biological Oxidation Systems. Volume 2
Consequently, 78% of the 15-HPE 2 would possess ls O-atoms in the hydroperoxide. Labeled 15-HPE2 was used as substrate for the oxidation of MPS to MPSO with purified enzyme. The PGE 2 resulting from this reaction (lower panel) showed 74% oxygen-18 at carbon 15 compared with 78% in the starting 15-HPE2. This excellent agreement confirmed that the hydroperoxide moiety was not exchanging in some unexpected fashion. The spectra of MPSO are on the right. On top is the spectrum of MPSO itself. The molecular ion at 140 is predominant with no 138 ion and only 5% the intensity at 142.
06 MHz in the Fourier transform mode. 12-Hz line broadening, 5-μΜ outer diameter sample tube, and 28°C probe temperature. 3 ppm, along with their integrals. Of course, other reducing substrates for the enzyme have also been examined. Of particular interest are studies by Marnett et al. that showed with diphenylisobenzofuran (DPBF) and phenylbutazone (PB) that the 1 8 0 in each product arose from dissolved molecular oxygen (12). With our knowledge that the sulfide was bound when oxidized, it seemed possible that we could distinguish the location of the primary oxidation of these molecules by looking for competition with the MPS (Table 2).
A. (1966) Reel. Trav. Chim. Pays-Bas 85,405 7. E. B. (1982) Mol. Pharmacol. 21,133 8. M. A. Jr. (1980) /. Biol Chem. 255,323 9. Tatum, CM. , Schleicher, E. G. (1977) Biochemistry 16,1093 10. H. Jr. H. (1962) J. Org. Chem. 27,1468 11. , Newman, N. M. (1962) J. Org. Chem. 27,2707 12. R. A. W. ) Vol. 6, pp. 149-151. Raven Press, New York Molecular Mechanisms for the Peroxidase Catalyzed Activation of Arylamine Carcinogens to Cytotoxic Metabolites PJ. O'Brien, S. Khan, S. G. McGirr Faculty of Pharmacy, University of Toronto 19 Rüssel Street, Toronto, Ontario M5S 2S2 Canada Abstract 1.